About the position
Research Assistant in Chaperone-Mediated Autophagy (CMA) and Experimental Biomedicine
We are seeking a research assistant with documented experience in chaperone-mediated autophagy (CMA) to join our research group at the Department of Physiology and Pharmacology, Karolinska Institutet. The work focuses on experimental assessment of CMA and lysosomal protein degradation using established functional reporter systems, cellular models and preclinical methods. You will contribute to ongoing studies in an international research environment where methodological expertise, quality and reproducibility are essential.
Your Mission
The role involves independently planning, performing and analysing experiments measuring CMA activity and lysosomal protein degradation. You will work with functional CMA reporter systems, including fluorescence-based reporters and quantitative reporter assays, and established cellular models to study CMA regulation. Responsibilities also include protein analysis, microscopy, experimental documentation and statistical data analysis. The position further involves participation in preclinical animal studies, including laboratory animal handling and collection and analysis of biological samples, in accordance with applicable permits, training requirements and procedures. You will contribute to method optimisation, quality control and reporting of research findings.
Your Profile
Required qualifications: A relevant university degree in biomedicine, cell biology or a related field. Documented hands-on research experience in chaperone-mediated autophagy (CMA), including independent use of functional CMA reporter systems and quantitative measurement of CMA activity. Experience with experimental cell models and assessment of lysosomal protein degradation. Documented practical experience handling laboratory mice and working in connection with preclinical animal experiments, with appropriate training and authorisation for the animal-related duties to be performed. Ability to independently plan, conduct, document and analyse experiments, and good written and spoken English. Meritorious qualifications: Experience with CMA-based fluorescent reporters and related cellular models, immunofluorescence and quantitative image analysis. Experience collecting and analysing tissue samples from animal studies, Western blotting, RT-qPCR and advanced statistical data analysis. Doctoral training or other advanced research experience in CMA and lysosomal biology.
What Do We Offer?
A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is also a state university, which entitles you to several good benefits through our collective agreement. And you get to practice freely in our modern wellness facilities, where trained staff are on site.
Location: Biomedicum, Solna
Department of Physiology and Pharmacology
Tagged as: Life Sciences
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