Research Specialist II (Ophthalmology)

Johns Hopkins, founded in 1876, is America's first research university and home to nine world-class academic divisions working together as one university.

We are seeking a Research Specialist II who will develop assays and protocols for PI approval and designs experimental approaches for laboratory projects. Performs a range of sophisticated assays. Makes significant contributions to various aspects of laboratory efforts and goals. Responsible for troubleshooting, independent decisions and training/guidance of other laboratory personnel.

Specific Duties & Responsibilities

• Conduct assigned laboratory projects.
• Utilize specialized knowledge to interpret results, e.g. read

controls, evaluate results, etc.

• Work independently and use scientific knowledge and lab

experience to make judgments and decisions.

• Teach/train learners, and new technicians in the

laboratory.

• Guide, direct, and facilitate problem solving for entry-level

laboratory members on day-to-day problems with more challenging research assays.

• Make scientific contributions to protocol development,

efficiency, and productivity.

• Collaborate in the development of new techniques and

instruments.

• Apply published research methods to current experimental work

by investigating published protocols and discussing them with
PI.

• Scientifically contribute to the preparation of manuscripts and

proposals.

• Process analyzed data into figures for scientific

publications.

• Order equipment and supplies, interface with vendors.
• Other duties as assigned.

We are seeking a highly motivated, energetic individual with an interest in biomedical research to work with human pluripotent stem cell. We use CRISPR genome editing to develop models of

• Human retinal diseases, with a focus on neurodegenerative

diseases such as glaucoma.

• Demyelinating diseases (such as multiple sclerosis where myelin

sheath on the CNS neurons is lost).

• Neurofibromatosis 1 (NF1), with a focus on the genetic

mutations in NF1 gene that cause dysfunction of glial cells such as astroglia, oligodendroglia and microglia.

• We also use mouse model of neurodegenerative diseases for drug

testing.

This work will involve a considerable amount of cell culture
(particularly human pluripotent stem cells) and molecular biology work.

• The successful applicant will work under the supervision of the

principal investigator and other laboratory personnel, and will be responsible for maintaining human pluripotent stem cells, as well as differentiating them into human retinal ganglion cells, oligodendrocytes, astrocytes, and microglia.

• They will also assist in various molecular biology work that

include molecular cloning, DNA/RNA extraction, PCR, qPCR, gel electrophoresis, and Western Blotting etc.

• While the majority of the work is related to cell culture and

molecular biology, some animal handling and tissue processing will also be required, which they will need to perform under the supervision of the principal investigator or a senior scientist.

• The position will also require good organization skill,

previous experience maintaining lab data, and working with vendors to order items and reagents for a research lab.

Minimum Qualifications

• Bachelor's Degree in Biology, Chemistry or related field.
• Two years of laboratory experience performing relevant

techniques.

• Additional education may substitute for required experience and

additional related experience may substitute for required education beyond a high school diploma/graduation equivalent, to the extent permitted by the JHU equivalency formula.

Preferred Qualifications

• Master's Degree with related graduate research.
• Some relevant post-undergraduate work experience in the field

of cellular and molecular biology.

• Experience with cell culture work, ideally with human stem cell

culture.

• Prior experience handling and working with animal models.

Technical Skills & Expected Level of Proficiency

• Assay Development and Validation - Developing
• Data Management and Analysis - Developing
• Experimental Design and Execution - Developing
• Operations Management - Developing
• Protocol Development and Optimization - Developing
• Scientific Writing - Developing
• Stakeholder Engagement - Developing

The core technical skills listed are most essential;
additional technical skills may be required based on specific division or department needs.

Classified Title: Research Specialist II

Role/Level/Range: ACRP/03/MB

Starting Salary Range: $42,330 - $74,110 Annually ($56,800 targeted; Commensurate w/exp.)

Employee group: Full Time

Schedule: Monday-Friday, 8:30 am - 5:00 pm

FLSA Status: Exempt

Location: School of Medicine Campus

Department name: SOM Oph Ocular Molecular Biology

Personnel area: School of Medicine

The successful candidate(s) for this position will be subject to a pre-employment background check.

If you are interested in applying for employment with The Johns
Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the
HR Business Services Office at jhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711.

The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.

During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against
Rubella (German measles), Rubeola (Measles), Mumps, Varicella
(chickenpox), Hepatitis B and documentation of having received the
Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

Equal Opportunity Employer

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