Research Assistant (Environmental Health and Engineering)

Johns Hopkins, founded in 1876, is America's first research
university and home to nine world-class academic divisions working
together as one university.

We are seeking a Research Assistant who will
oversee data collection, data organization, and/or data management
or similar functions/tasks for research study(ies) in support of a
PI or a research team.

Specific Duties & Responsibilities

• Run routine and ad hoc reports.
• Use standard tools and computer programs to review data.
• Assist with data cleaning measures to ensure accuracy of data

and preparation of tables.

• Lead basic activities such as data collection and data

entry.

• May lead specific tasks and develop processes to ensure study

activities occur effectively and efficiently.

• May conduct literature searches to support faculty in research

efforts.

• May design and format papers/publications.
• May assist PIs in writing summaries of papers for release as

policy briefs or other channels.

• Other duties as assigned.

In Addition to the Duties Listed Above

• The Center for Alternatives to Animal Testing (CAAT),

Department of Environmental Health and Engineering, Johns Hopkins
Bloomberg School of Public Health, is seeking a Research Assistant
to support laboratory research focused on the development of
alternatives to animal testing.

• The main area of research in the laboratory is neurotoxicity

and developmental neurotoxicity, with links to autism and
neurodegeneration.

• The research is focused entirely on in vitro methods — in

particular, the development of in vitro 3D brain organotypical
cultures (brain organoids), iPSC culture, electrophysiiology, and
toxicity testing in brain organoids. The laboratory offers a
collaborative, international research environment.

The Research Assistant will work closely with PhD
students and postdoctoral fellows in the lab, taking care of iPSC,
neuroprogenitor cells, and 3D brain organoid cultures, and will be
responsible for the general operation and maintenance of the
laboratory. The Research Assistant will

• Maintain iPSC, neuroprogenitor cells, and 3D brain organoid

cultures, including thawing, passaging, differentiation,
cryopreservation, and routine quality control.

• Perform general maintenance of the laboratory, including cell

culture work and keeping shared culture areas and equipment in
working order.

• Perform immunohistochemistry, RNA isolation, and Real-Time PCR,

and support related molecular and cell biology assays.

• Prepare media, buffers, reagents, and solutions, and maintain

accurate preparation records.

Minimum Qualifications

• Bachelor's Degree in a related field.
• Additional education may substitute for required experience and

additional related experience may substitute for required education
permitted by the JHU equivalency formula beyond a high school
diploma/graduation equivalent, to the extent of the JHU equivalency
formula.

Preferred Qualifications

• Bachelor's or Master's Degree in Biology, Biochemistry,

Pharmacology, Neuroscience, or a related discipline.

• One year of work experience in a cell culture laboratory.
• Cell culture experience (stem cells and organoids in

particular), aseptic technique, and basic wet laboratory operations
such as RNA isolation, Real-Time PCR, and Western blot.

• Hands-on experience with human induced pluripotent stem cell

(iPSC) culture, including maintenance, differentiation, and quality
control.

• Solid working knowledge of molecular biology, including nucleic

acid and protein techniques.

• Experience with gene editing techniques, such as cloning and

CRISPR/Cas9 design, delivery, and clone validation.

• Knowledge of microphysiological systems (MPS), organ-on-chip

platforms, or other 3D in vitro model systems.

• Background or coursework in neuroscience or developmental

neurobiology and electrophysiology.

• Careful and accurate record keeping, strong organizational

skills, and the ability to work both independently and as part of a
diverse, multidisciplinary team.

Technical Skills & Expected Level of Proficiency

• Analytical Skills - Awareness
• Attention to Detail - Awareness
• Data Management and Analysis - Awareness
• Formatting and Layout Proficiency - Awareness
• Information Gathering - Awareness
• Oral and Written Communications - Awareness
• Organizational Skills - Awareness

The core technical skills listed are most essential;
additional technical skills may be required based on specific
division or department needs.

Classified Title: Research Assistant

Role/Level/Range: ACRO40/E/03/CD

Starting Salary Range: $17.65 - $31.05 HRLY (Commensurate
w/exp.)

Employee group: Full Time

Schedule: Monday to Friday: 9:00am – 5:00pm

FLSA Status: Non-Exempt

Location: School of Public Health

Department name: Environmental Health and Engineering

Personnel area: School of Public Health

The successful candidate(s) for this position will be subject to a
pre-employment background check.

If you are interested in applying for employment with The Johns
Hopkins University and require special assistance or accommodation
during any part of the pre-employment process, please contact the
HR Business Services Office at jhurecruitment@jhu.edu. For TTY
users, call via Maryland Relay or dial 711.

The following additional provisions may apply depending on which
campus you will work. Your recruiter will advise
accordingly.

During the Influenza ("the flu") season, as a condition of
employment, The Johns Hopkins Institutions require all employees
who provide ongoing services to patients or work in patient care or
clinical care areas to have an annual influenza vaccination or
possess an approved medical or religious exception. Failure to meet
this requirement may result in termination of employment.

The pre-employment physical for positions in clinical areas,
laboratories, working with research subjects, or involving
community contact requires documentation of immune status against
Rubella (German measles), Rubeola (Measles), Mumps, Varicella
(chickenpox), Hepatitis B and documentation of having received the
Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include
documentation of having two (2) MMR vaccines; two (2) Varicella
vaccines; or antibody status to these diseases from laboratory
testing. Blood tests for immunities to these diseases are
ordinarily included in the pre-employment physical exam except for
those employees who provide results of blood tests or immunization
documentation from their own health care providers. Any
vaccinations required for these diseases will be given at no cost
in our Occupational Health office.

Equal Opportunity Employer

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